Biological soft tissue characterisation is a multidisciplinary field vital for biomedical applications, from device design to disease treatment. Soft tissues exhibit complex multiscale structures and multiphysics interactions. This colloquium brings together experts in biomechanics, materials science, and computational modelling to advance soft tissue characterisation. Key discussions will explore how tissue mechanics, from cells to organs, influence tissue behaviour, improvements in experimental data acquisition, and the development of advanced constitutive models for complex tissue properties. A major focus is translating research into clinical applications, such as better diagnostic tools and treatments. The event also addresses remaining challenges and promotes interdisciplinary collaboration to accelerate progress and bridge gaps between theory and practice.